It has been shown that the strength of spin-spin interactions in a spin-1/2 chain can be evaluated through one of the edge spins only. We experimentally demonstrate this for a three-spin chain with NMR techniques. The three spins in the chain interact through the nearest-neighbor Ising-type interactions under site-dependent transverse fields. The molecule employed is alanine containing three 13C nuclei, each of which has spin-1/2.